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| Registros recuperados: 44 | |
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| Bellec, Laure; Cambon-bonavita, Marie-anne; Cueff-gauchard, Valerie; Durand, Lucile; Gayet, Nicolas; Zeppilli, Daniela. |
| Deep-sea hydrothermal vent meiofauna have been the focus of recent research and the discovery of an abundant well-adapted free-living marine nematode on the Mid-Atlantic Ridge offers new perspectives on adaptations to the vent environment. Indeed, knowledge concerning biological interactions of microbes and meiofauna in marine extreme environments is scarce, especially for nematodes. In this study, we used microscopic observations [fluorescence in situ hybridization (FISH) and scanning electron microscopy (SEM)] and metabarcoding of 16S rRNA to characterize the bacterial community of the nematode species Oncholaimus dyvae, an overlooked but ecologically important vent organism. Detection of bacteria in the buccal cavity and on the cuticle (SEM) and... |
| Tipo: Text |
Palavras-chave: Sulfur-oxidizing bacteria; Lucky Strike vent field; Meiofauna; Nematode; Endosymbiont. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00457/56846/58676.pdf |
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| Guri, Mathieu; Durand, Lucile; Cueff-gauchard, Valerie; Zbinden, Magali; Crassous, Philippe; Shillito, Bruce; Cambon-bonavita, Marie-anne. |
| The caridean shrimp Rimicaris exoculata dominates the fauna at several Mid-Atlantic Ridge hydrothermal vent sites. This shrimp has an enlarged gill chamber, harboring a dense ectosymbiotic community of chemoautotrophic bacteria associated with mineral oxide deposits. Until now, their acquisition is not fully understood. At three hydrothermal vent sites, we analyzed the epibionts diversity at different moult stages and also in the first stages of the shrimp life (eggs, hatched eggs (with larvae) and juveniles). Hatched eggs associated with young larvae were collected for the first time directly from gravid females at the Logachev vent site during the Serpentine cruise. An approach using 16S rRNA clone libraries, scanning and transmission electron... |
| Tipo: Text |
Palavras-chave: Symbiosis; Larvae; Methanotrophic symbiont; Rimicaris exoculata; Transmission pathways. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00072/18318/15997.pdf |
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| Le Bloa, Simon; Boidin-wichlacz, Céline; Cueff-gauchard, Valerie; Rosa, Rafael Diego; Cuvillier-hot, Virginie; Durand, Lucile; Methou, Pierre; Pradillon, Florence; Cambon-bonavita, Marie-anne; Tasiemski, Aurélie. |
| The symbiotic shrimp Rimicaris exoculata dominates the macrofauna inhabiting the active smokers of the deep-sea mid Atlantic ridge vent fields. We investigated the nature of the host mechanisms controlling the vital and highly specialized ectosymbiotic community confined into its cephalothoracic cavity. R. exoculata belongs to the Pleocyemata, crustacean brooding eggs, usually producing Type I crustins. Unexpectedly, a novel anti-Gram-positive type II crustin was molecularly identified in R. exoculata. Re-crustin is mainly produced by the appendages and the inner surfaces of the cephalothoracic cavity, embedding target epibionts. Symbiosis acquisition and regulating mechanisms are still poorly understood. Yet, symbiotic communities were identified at... |
| Tipo: Text |
Palavras-chave: Extreme; Hydrothermal; Symbiosis; Host-microbe interaction; Invertebrate immunity; Crustacean. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00643/75534/76413.pdf |
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| Vigneron, Adrien; Cruaud, Perrine; Pignet, Patricia; Caprais, Jean-claude; Gayet, Nicolas; Cambon-bonavita, Marie-anne; Godfroy, Anne; Toffin, Laurent. |
| The Sonora Margin cold seeps present on the seafloor a patchiness pattern of white microbial mats surrounded by polychaete and gastropod beds. These surface assemblages are fuelled by abundant organic inputs sedimenting from the water column and upward-flowing seep fluids. Elevated microbial density was observed in the underlying sediments. A previous study on the same samples identified anaerobic oxidation of methane (AOM) as the potential dominant archaeal process in these Sonora Margin sediments, probably catalysed by three clades of archaeal anaerobic methanotrophs (ANME-1, ANME-2 and ANME-3) associated with bacterial syntrophs. In this study, molecular surveys and microscopic observations investigating the diversity of Bacteria involved in AOM... |
| Tipo: Text |
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| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00168/27921/27279.pdf |
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| Corbari, Laure; Zbinden, Magali; Cambon-bonavita, Marie-anne; Gaill, Françoise; Compere, Philippe. |
| The shrimp Rimicaris exoculata is considered a primary consumer that dominates the fauna of most Mid-Atlantic Ridge hydrothermal ecosystems. The shrimps harbour in their gill chamber an important ectosymbiotic community of chemoautotrophic bacteria associated with iron oxide deposits. The settlement and development of this ectosymbiosis was investigated using microscopy techniques (light microscopy, LM; and scanning, transmission and environmental scanning electron microscopy: SEM and ESEM, respectively) for shrimps from 2 different vent fields (Rainbow, 36 degrees 14.0' N and TAG, 26 degrees 08.0' N). The results revealed a bacterial re-colonisation after each exuviation and a development of the bacterial community in 5 steps in relation to the moult... |
| Tipo: Text |
Palavras-chave: Iron oxides; Ectosymbiosis; Moult cycle; Shrimp; Hydrothermal vents. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4626.pdf |
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| Durand, Lucile; Roumagnac, Marie; Cueff-gauchard, Valerie; Jan, Cyrielle; Guri, Mathieu; Tessier, Claire; Haond, Marine; Crassous, Philippe; Zbinden, Magali; Arnaud-haond, Sophie; Cambon-bonavita, Marie-anne. |
| Rimicaris exoculata is a deep-sea hydrothermal vent shrimp which enlarged gill chamber houses a complex trophic epibiotic community. Its gut harbours an autochthonous and distinct microbial community. This species dominates hydrothermal ecosystems megafauna along the Mid-Atlantic Ridge, regardless of contrasted geochemical conditions prevailing in them. Here, the resident gut epibiont community at four contrasted hydrothermal vent sites (Rainbow/TAG/Logatchev/Ashadze) was analysed and compiled with previous data to evaluate the possible influence of site location, using 16S rRNA surveys and microscopic observations (TEM, SEM and FISH analyses). Filamentous epibionts inserted between the epithelial cells microvilli were observed on all examined samples.... |
| Tipo: Text |
Palavras-chave: Epibiont; Extreme environments; Network analyses; Phylogeography. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00288/39890/38443.pdf |
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| Wery, Nathalie; Moricet, Jean-marc; Cueff, Valerie; Jean, Joelle; Pignet, Patricia; Lesongeur, Francoise; Cambon-bonavita, Marie-anne; Barbier, Georges. |
| A thermophilic, anaerobic, chemo-organotrophic bacterium, designated MV1087(T), was isolated from a deep-sea hydrothermal chimney sample collected from the Mid-Atlantic Ridge. The cells were straight, motile and stained Gram-negative. Growth was observed from 45 to 65 degreesC, with an optimum around 65 degreesC. No growth was observed at 40 or 70 degreesC. Growth was observed from pH 5.5 to 9.0 and the optimum pH was around 7. The salinity range for growth was 10-100 g sea salt l(-1) (corresponding to 6.5-65 g NaCl l(-1)) with an optimum at 30 g sea salt l(-1) (20 g NaCl l(-1)). Strain MV1087(T) was heterotrophic, able to ferment proteinaceous substrates, such as brain/heart infusion and gluten, and carbohydrates, such as glucose, xylan and starch. The... |
| Tipo: Text |
Palavras-chave: Thermophile; Deep sea vent; Caloranaerobacter azorensis; Caloranaerobacter; Bacteria. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/publication-586.pdf |
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| Alain, Karine; Pignet, Patricia; Zbinden, M; Quillevere, M; Duchiron, F; Donval, Jean-pierre; Lesongeur, Francoise; Raguenes, Gerard; Crassous, Philippe; Querellou, Joel; Cambon-bonavita, Marie-anne. |
| A novel thermophilic, anaerobic, strictly chemoorganoheterotrophic bacterium, designated as AM1114(T), was isolated from a deep-sea hydrothermal vent sample from the East-Pacific Rise (EPR 13degrees N). The cells were long (3-10 mum) rods, motile with peritrichous flagella, and exhibited a Gram-negative cell wall ultrastructure. In the late stationary phase of growth, cells formed an ovoid, refractile, terminal endospore. They grew at 45-65 degreesC inclusive (optimum 55-60 degreesC; doubling time approx. 45 min), at pH 4.5-8.0 inclusive (optimum pH 7.5-8.0) and at sea salt concentrations of 20-60 g l(-1) inclusive (optimum 25-30 g l(-1)). Strain AM1114(T) was an obligately heterotrophic bacterium able to ferment a mixture of 20 amino acids, complex... |
| Tipo: Text |
Palavras-chave: Caminicella sporogenes; Clostridiales; Thermophile; Deep sea hydrothermal vent. |
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/2002/publication-528.pdf |
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| Jiang, Lijing; Liu, Xuewen; Dong, Chunming; Huang, Zhaobin; Cambon-bonavita, Marie-anne; Alain, Karine; Gu, Li; Wang, Shasha; Shao, Zongze. |
| The deep-sea hydrothermal vent shrimp Rimicaris exoculata largely depends on a dense epibiotic chemoautotrophic bacterial community within its enlarged cephalothoracic chamber. However, our understanding of shrimp-bacterium interactions is limited. In this report, we focused on the deltaproteobacterial epibiont of R. exoculata from the relatively unexplored South Mid-Atlantic Ridge. A nearly complete genome of a Deltaproteobacteria epibiont was binned from the assembled metagenome. Whole-genome phylogenetic analysis reveals that it is affiliated with the genus Desulfobulbus, representing a potential novel species for which the name "Candidatus Desulfobulbus rimicarensis" is proposed. Genomic and transcriptomic analyses reveal that this bacterium utilizes... |
| Tipo: Text |
Palavras-chave: Deltaproteobacteria; Rimicaris exoculata; Wood-Ljungdahl pathway; Epibiont; Sulfur disproportionation. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00624/73600/76007.pdf |
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| Laming, Sven; Hourdez, Stéphane; Cambon-bonavita, Marie-anne; Pradillon, Florence. |
| The chemosymbiotic gastropod Alviniconcha (Provannidae), first described in 1988, is one of the most emblematic hydrothermal-vent taxa described from the Central Indian Ridge and the Southwest (SW) Pacific. Symbiotic bacteria found in the gill of Alviniconcha are thought to be their principal source of nutrition. In the SW Pacific, species distributions for A. kojimai, A. boucheti – and to a lesser extent A. strummeri – overlap. While Alviniconcha species do not appear to truly co-exist in these highly energetic but spatially limited habitats, certain species regularly co-occur within a single vent field and in rare instances, the same edifice. Past research suggests that SW-Pacific Alviniconcha species might aggregate around fluids with distinct... |
| Tipo: Text |
Palavras-chave: Deep sea; Chemosymbiotic; Periostracum; Gastropod; Habitat partitioning; Computed tomography; Histology; Taxonomy; 3D model. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00628/74048/73459.pdf |
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| Cruaud, Perrine; Vigneron, Adrien; Pignet, Patricia; Caprais, Jean-claude; Lesongeur, Francoise; Toffin, Laurent; Godfroy, Anne; Cambon-bonavita, Marie-anne. |
| In the Guaymas Basin, the presence at a few tens of kilometers of cold seeps and hydrothermal vents coupled with comparable sedimentary settings and depths offer a unique opportunity to assess and compare the microbial community composition of these deep-sea ecosystems. The microbial diversity in sediments from three cold seep and two hydrothermal vent assemblages were investigated using high-throughput 16S rRNA-sequencing. Numerous bacterial and archaeal lineages were detected in both cold seep and hydrothermal vent sediments. Various potential organic matter degraders (e.g., Chloroflexi, Atribacteria, MBG-D) and methane and sulfur cycling related microorganisms (e.g., ANME and methanogenic lineages, sulfate-reducing lineages) were detected in both... |
| Tipo: Text |
Palavras-chave: Microbial diversity; Hydrothermal vents; Cold seeps; Ribotypes; Methane; Extreme environments. |
| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00415/52640/53496.pdf |
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| Roussel, Erwan; Konn, Cecile; Charlou, Jean-luc; Donval, Jean-pierre; Fouquet, Yves; Querellou, Joel; Prieur, Daniel; Cambon-bonavita, Marie-anne. |
| The distribution of Archaea and methanogenic, methanotrophic and sulfate-reducing communities in three Atlantic ultramafic-hosted hydrothermal systems (Rainbow, Ashadze, Lost City) was compared using 16S rRNA gene and functional gene (mcrA, pmoA and dsrA) clone libraries. The overall archaeal community was diverse and heterogeneously distributed between the hydrothermal sites and the types of samples analyzed (seawater, hydrothermal fluid, chimney and sediment). The Lost City hydrothermal field, characterized by high alkaline warm fluids (pH>11; T<95 °C), harbored a singular archaeal diversity mostly composed of unaffiliated Methanosarcinales. The archaeal communities associated with the recently discovered Ashadze 1 site, one of the deepest active... |
| Tipo: Text |
Palavras-chave: Archaea; Hydrothermal vent; Mid-Atlantic Ridge; 16S rRNA gene; Sediment; Ultramafic. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00067/17836/15573.pdf |
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| Gaill, Françoise; Ballu, V.; Cannat, M.; Crawford, W.; Dyment, J.; Escartin, J.; Fouquet, Yves; Goslin, J.; Reverdin, G.; Sarradin, Pierre-marie; Tarits, P.; Andreani, M.; Bonnivard, E.; Bucas, Karenn; Burgaud, G.; Cambon-bonavita, Marie-anne; Cueff, Valerie; Durand, C.; Gros, O.; Hamel, G.; Henriques, M.; Hois, E.; Ildefonse, B.; Konn, Cecile; Le Bris, Nadine; Le Guyader, H.; Ravaux, J.; Shilito, B.; Toullec, J.y.; Zbinden, M.. |
| Tipo: Text |
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| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/00385/49650/50163.pdf |
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| Alain, Karine; Rolland, Sophie; Crassous, Philippe; Lesongeur, Francoise; Zbinden, Magali; Le Gall, Christian; Godfroy, Anne; Page, Antoine; Juniper, S Kim; Cambon-bonavita, Marie-anne; Duchiron, Francis; Querellou, Joel. |
| A novel thermophilic, chemolithoautotrophic T bacterium, designated as NE1206(T), was isolated from a Juan de Fuca Ridge hydrothermal vent sample (tubes of the annelid polychaete Paralvinella sulfincola attached to small pieces of hydrothermal chimney). The cells were rod-shaped (1.2-3.5x0.4-0.7 mum), occurring as single motile rods or forming macroscopic aggregates visible as pinkish to brownish streamers. The new isolate was anaerobic. It grew between 50 and 70 degreesC (optimum 60-65 degreesC doubling time approximately 1 h 15 min at 60 degreesC), between pH 5.0 and 7.5 (optimum pH around 6.0-6.5) and at sea salts concentrations between 20 and 40 g l(-1) (optimum 30 g l(-1)). Cells grew chemolithoautotrophically in an H-2/CO2 atmosphere (80/20, v/v; 200... |
| Tipo: Text |
Palavras-chave: Thermophile; Nitrate reducing; Lithotroph; Desulfurobacterium crinifex; Deep sea hydrothermal vent; Cell networks. |
| Ano: 2003 |
URL: https://archimer.ifremer.fr/doc/2003/publication-663.pdf |
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| Auguste, M.; Mestre, N. C.; Rocha, T. L.; Cardoso, C.; Cueff-gauchard, Valerie; Le Bloa, Simon; Cambon-bonavita, Marie-anne; Shillito, B.; Zbinden, M.; Ravaux, J.; Bebianno, M. J.. |
| In light of deep-sea mining industry development, particularly interested in massive-sulphide deposits enriched in metals with high commercial value, efforts are increasing to better understand potential environmental impacts to local fauna. The aim of this study was to assess the natural background levels of biomarkers in the hydrothermal vent shrimp Rimicaris exoculata and their responses to copper exposure at in situ pressure (30 MPa) as well as the effects of depressurization and pressurization of the high-pressure aquarium IPOCAMP. R. exoculata were collected from the chimney walls of the hydrothermal vent site TAG (Mid Atlantic Ridge) at 3630 m depth during the BICOSE cruise in 2014. Tissue metal accumulation was quantified in different tissues... |
| Tipo: Text |
Palavras-chave: Deep-sea mining; Hydrothermal vents; Biomarkers; Ipocamp; Copper; TAG. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00323/43465/42869.pdf |
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| Cambon-bonavita, Marie-anne; Nadalig, Thierry; Roussel, Erwan; Delage, Eloise; Duperron, Sebastien; Caprais, Jean-claude; Boetius, A.; Sibuet, Myriam. |
| A giant 800-m-diameter pockmark named REGAB was discovered on the Gabon continental margin actively emitting methane at a water depth of 3200 m. The microbial diversity in sediments from four different assemblages of chemosynthetic organisms, Mytilidae, Vesicomyidae, Sibogliniclae and a bacterial mat, was investigated using comparative 16S rRNA gene sequence analysis. Aggregates of anaerobic methanotrophic archaea (ANME-2) and bacteria of the Desulfosarcina/Desulfococcus cluster were found in all four chemosynthetic habitats. Fluorescence in situ hybridization targeting the ANME-2/Desulfosarcina/Desulfococcus aggregates showed their presence few centimeters (3-5cm) below the surface of sediment. 16S rRNA gene sequences from all known marine ANME groups... |
| Tipo: Text |
Palavras-chave: Microbial phylogeny; Faunal assemblage; Cold seep; Chemosynthetic ecosystems; AOM; REGAB. |
| Ano: 2009 |
URL: https://archimer.ifremer.fr/doc/2009/publication-7298.pdf |
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| Zeppilli, Daniela; Bellec, Laure; Cambon-bonavita, Marie-anne; Decraemer, Wilfrida; Fontaneto, Diego; Fuchs, Sandra; Gayet, Nicolas; Mandon, Perrine; Michel, Loic; Portail, Marie; Smol, Nic; Sørensen, Martin V.; Vanreusel, Ann; Sarrazin, Jozee. |
| Background Nematodes are an important component of deep-sea hydrothermal vent communities, but only few nematode species are able to cope to the harsh conditions of the most active vent sites. The genus Oncholaimus is known to tolerate extreme geothermal conditions and high sulphide concentrations in shallow water hydrothermal vents, but it was only occasionally reported in deep-sea vents. In this study, we performed morphological, genetic and ecological investigations (including feeding strategies) on an abundant species of Oncholaimus recently discovered at Lucky strike vent field on the Mid-Atlantic Ridge at 1700 m water depth. Results We described this species as Oncholaimus dyvae sp. nov.. This new species differs from all other members of the genus... |
| Tipo: Text |
Palavras-chave: Oncholaimus dyvae sp. nov.; Hydrothermal vents; Deep sea; Carbon and nitrogen isotopic ratios. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00508/61985/66097.pdf |
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| Brandt, Miriam; Pradillon, Florence; Trouche, Blandine; Henry, Nicolas; Liautard-haag, Cathy; Cambon-bonavita, Marie-anne; Cueff-gauchard, Valerie; Wincker, Patrick; Belser, Caroline; Poulain, Julie; Arnaud-haond, Sophie; Zeppilli, Daniela. |
| Despite representing one of the largest biomes on earth, biodiversity of the deep seafloor is still poorly known. Environmental DNA metabarcoding offers prospects for fast inventories and surveys, yet requires standardized sampling approaches and careful choice of environmental substrate. Here, we aimed to optimize the genetic assessment of prokaryote (16S), protistan (18S V4), and metazoan (18S V1–V2, COI) communities, by evaluating sampling strategies for sediment and aboveground water, deployed simultaneously at one deep-sea site. For sediment, while size-class sorting through sieving had no significant effect on total detected alpha diversity and resolved similar taxonomic compositions at the phylum level for all markers studied, it effectively... |
| Tipo: Text |
Palavras-chave: Biodiversity; Ecological genetics; Microbial ecology; Molecular ecology. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00689/80094/83149.pdf |
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| Roussel, Erwan; Cambon-bonavita, Marie-anne; Querellou, Joel; Cragg, B; Webster, G; Prieur, D; Parkes, R. |
| Sub-sea-floor sediments may contain two-thirds of Earth's total prokaryotic biomass. However, this has its basis in data extrapolation from ~500-meter to 4-kilometer depths, whereas the deepest documented prokaryotes are from only 842 meters. Here, we provide evidence for low concentrations of living prokaryotic cells in the deepest (1626 meters below the sea floor), oldest (111 million years old), and potentially hottest (~100°C) marine sediments investigated. These Newfoundland margin sediments also have DNA sequences related to thermophilic and/or hyperthermophilic Archaea. These form two unique clusters within Pyrococcus and Thermococcus genera, suggesting unknown, uncultured groups are present in deep, hot, marine sediments (~54° to 100°C). Sequences... |
| Tipo: Text |
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| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4209.pdf |
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| Sarradin, Pierre-marie; Sarrazin, Jozee; Cadiou, Jean-francois; Olu, Karine; Desbruyeres, Damien; Rigaud, Vincent; Drogou, Jean-francois; Lecornu, Fabrice; Rolin, Jean-francois; Vuillemin, Renaud; Cambon-bonavita, Marie-anne; Sauter, E; Von Juterzenka, K; Boetius, A; Santos, Rs; Colaco, Ana; Pascoal, A; Oliveira, Paulo; Shillito, Bruce; Zal, F; Schultz, A; Taylor, P; Lane, D; Loke, Rob; Du Buf, H; Waldmann, C; Cormack, A; Sanfilippo, L; Masson, M. |
| The general objective of EXOCET/D is to develop, implement and test specific instruments aimed at exploring, describing, quantifying and monitoring biodiversity in deep-sea fragmented habitats as well as at identifying links between community structure and environmental dynamics. Onboard experimental devices will complement the approach, enabling experiments on species physiology. The EXOCET/D working fields include: video and acoustic imagery, in situ analysis of physico-chemical factors, quantitative sampling of macroand micro-organisms, in vivo experiments, integration of multidisciplinary data, implementation on European deep-submersibles and a final phase of technical and scientific validation |
| Tipo: Text |
Palavras-chave: Deep sea; Ecosystem; Imagery; In situ analysis; Faunal sampling; Submersibles; Observatory. |
| Ano: 2004 |
URL: https://archimer.ifremer.fr/doc/2007/publication-3600.pdf |
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| Registros recuperados: 44 | |
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